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PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATlC HYBRIDIZATION
EXPRESSIONOFHUMANINTERLEUKIN-1BGENEINTRANSGENIC
HAIRY ROOTCULTURE OFHORSERADISH (ARMORАСI A
RUSTICANA)
N.S.Lyapkova, A.A.Shadenkov, A.N.Maisuryan, M.V. Kovalyova,
M.F.Shemyakin
Research Institute o fAgricultural Biotechnology, RAAS, Moscow, Russia, E-Mail:
Hum an interleukin-1 β (IL-1β) concerns to cytokines - the universal
imm une response proteins. The IL-1β is known to excret into intracellular
space in human blood. The protein is activated with the help of specific
proteinase o f unknown other properties. In this study the expression o f IL-1 β
in transgenic hairy root culture of horseradish w as investigated. The developed
system o f cultivating o f horseradish in liquid medium is convenient for the
study o f protein excretion and the accumulation o f preparative scales of
proteins. The designed gene where the IL-1 p sequence is under the control
o f plant 35S -prom oter, was entered Into the structure o f binary vector.
T ra n s g e n ic h o rs e ra d is h p la n ts w ith th is gene w e re o b ta in e d a fte r
transformation of Agrobacterium tumefaciens . The subsequent transformation
o f these transgenic plants with the help o f Agrobacterium rhizogenes A4
strain resulted in hairy root culture where IL-1 β is expressed. The data of
imm unoblotting assay specify that the product of IL-1β gene is a 31 kD
protein. So, it corresponds to the size o f prointerleukin. Obviously, the
proteinase specific to full-length IL-1 β gene product is a b s e n t in higher plants.
Nowadays we determine the level of IL-1 β in media where hary root iscultivated.
imm unoassay techniques are used for this aim .
This w o rk is su p p o rte d by Russian Founda tion o f F undam en ta l
Investigations.
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