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The influence of led and laser radiation on the growth and propagation of berry
cultures
in vitro
on the example of black raspberry and actinidia kolomikta
Solovykh N., Budagovsky A., Yankovskaya M.
The article presents results of research on effect of the additional laser and led lighting on the growth
and propagation of berry cultures
in vitro
. The relevance of the work due to the need of improving of methods
of clonal micropropagation of plants and the creation of energy-saving technologies of their cultivation.
The aim of this work was to study the effects of additional red light on the growth and reproduction of
plants cultivated
in vitro
at different levels of basic polychromatic light. Microshoots of black raspberry (
Rubus
occidentalis
L.) and Actinidia kolomikta (
Actinidia kolomikta
Maxim.) cultivated under basic illumination
1250 and 2500 Lux, were subjected to an additional daily lighting with led-emitting diodes (5 W/m
2
, 60
minutes) or helium-neon laser (15 W/m
2
, 8 minutes)) with wavelengths that cause the transition of
phytochromes in active conformation state (638 nm, 632,8 nm).
In low-light conditions (1250 Lux) the decrease of growth and propagation of black raspberry
compared with those with illumination 2500 lux was noted. For Actinidia kolomikta illumination suite
1250 lux proved to be optimal. Supplementary lighting of light-emitting diodes (LED) and laser increased
the propagation and the length of shoots of berry plants
in vitro
. The greatest stimulating effect of red
lighting is marked in a low base light (1250 Lux). In black raspberry laser or LED supplementary light-
ing allowed in low light (1250 Lux) to get the coefficient of multiplication and length of microshoots, not
statistically different from those in full light (2500 Lux) without supplementary lighting. Laser irradiation
stimulated the growth and propagation of plants more effectively than LED, probably because of greater
coherence.
Key words:
berry plants, clonal micropropagation in vitro, photoregulatory stimulation, system of
phytochromes, black raspberry, actinidia kolomikta
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