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The influence of polystimulines (PS) A6 and К on germination of several plant
seeds was investigated. The seeds of several plant species were placed on filter paper
imbibed with deionized water (control) or NaCI 155 mM and .NaCI 155 mM plus
PS-A6 or PS-K of following concentrations: 10,20,30,40,50,60 mg/I .It is established,
that sodium chloride inhibited seed germination, almost at all plant species. However,
at
G kiiitchia (riacanthos
L. and
Robinia pseudoacacia
L. germinated 10-15% of
seeds, Nevertheless in salinity conditions percentage of germination seeds at species
which was treatment of polystimulines amount 30 %. The determination of the ion
concentrations Na\K*,Cl' indicated that the sodium accumulation was similar in
seedlings of both species and, in general, Na+ was confined to the root. Chloride
accumulated at high levels in both root and shoot but least in
Gleditchia triacam hos.
The positive effects of polystimulines A6 and К on germination, sprouting leaves
and corm production in
Crocus sativus
L. are observed.
We found that the effect of polystimulines on germination of seeds depend from
their concentrations and plant species. In series experiments it was determined that for
seeds of forest plants optimal concentration of polystimulines is 50 mg/I and for seeds
of agricultural plants were determined 30 mg/I.
Take into consideration of breeding results can be observed that the germinating of
seeds and growth third old of wheat seedling under the low temperature (10°C) no so
much lowering visible growth have much is leaded to change of balance
phytohormones and redistribution them in organs of seedlings. It is believed that
ABA has an executing role as stress hormone signal for roots causing increasing its
autograph synthesis in roots.
At the mean salinity of 155 mM NaCI showed significally lower intensity of
photosynthesis , transpiration and plant growth (
Oossipium hirsutum
L.). Nevertheless,
exogenous treatment of PS-Аб promoting stimulation of vital activity of plants at
early and following stages under mean salinity of 51 and 103 mM NaCI. Antistress
effect of PS-A6 in. salty conditions is expressed by enchancement of the activity of
photosynthetic apparatus and increase of intensity of growth and productivity of
plants.
In other experiments were investigated the effect of polystimuline К (PS-K) on
photosynthetic activity of salt stressed
Trianea bogotensis
Karst. Chlorophyll variable
fluorescence (Fv) and slow fluorescence (SF) have been used to define the sensitivity
of photosystem II (PS 11) to different salt stress treatments (103,155 and344 mM),
at different time courses (15,30,60,360 and 720 minutes). Salt stress (155 mM NaCI)
treatments of the seedlings inhibited the SF magnitude approximately to 50%.
Preatreatment of seedlings for 30 minutes in 20 mg/I PS-K followed by salt stress
resulted in an increase in SF. magnitude. Under salt stress the magnitude of Fv/Fo
decreases within all time measurments. In the presence of NaCI and PS-K until 60
minutes of treatment there was a decrease in Fv/F0 value, then a slight increase was
observed. These results indicate that in
Trianea bogotensis
Karst, subjected to severe
salt stress the non*siomata! component of photosynthesis was affected and perhaps a
light-dependent inactivation of the primary photochemistry associated with photosystem
11(PS 11) occurred. The reduced efficiency of the NaCI effect in the
RESULTS AND DISCUSSION
147
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