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Т О T IP O T E N C Y A N D M O R P H O G E N E S IS OF P L A N T C E L L S
IN V IT R O
SCREENING OFPEA
(PISUMSATIVUML .)
COLLECTION TO
MORPHOGENETIC CAPACITYIN TISSUECULTURE
T.V.Jarkova,E.V.Deineko, V.K.Shumny
Institute o fCytology and Genetics ofSiberianBranch ofAcademy ofSciences, Russia,
630090. c. Novosibirsk-90, Lavrentiev av. 10, tel:(3832) 356-135,fax:(3832) 356-558,
Morphogenetic capacity of 26 genotypes of pea was estimated in tissue
culture on two protocols of media. The samples of pea were compare in
callus induction and capacity to morphogenetic responses. There was
considerable variation in time occurrence of callus formation (5-12 days after
beginning of cultivation). Some variation in frequency of callus formation and
relative growth of it was noted. The most part o f samples tested was
characterised by 100% callus formation. Among 26 genotypes investigated
12 had morphgenetic responses on one or both protocols of media. Variation
in regeneration time occurrence was 13-35 days depending on protocols
used. S ignificant differences have been observed in frequency of
organogenesis and somatic embryogenesis. Variety SIR-5 and line 6-14
gave a much better response than other genotypes tested for organogenesis
and somatic embryogenesis. Frequency of organogenesis was 75,2±1,1%
(SIR-5) and 58±1,0% (6-14), and of somatic embryogenesis was 79,3±2,0%
(SIR-5) and 70,8±1,4% (6 -14 ).
The present investigation confirmed the influence of genotype to
organogenesis and somatic embryogenesis. Samples of high regeneration
competence in tissue culture have been revealed.
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