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TO T IPO T EN C Y AND M O R PH O G EN E SIS O F PLANT C E L L S

IN V IT R O

INFLUENCEOFPHYSICALACTIONSONREGENERATIVEABILITYOF

UNFERTILIZEDOVULESOFSUGARBEET

V.V.Snamenсkaja, O.A.Podvigina,N.A.Taratonov

TheAll-RussianResearch Institute o fSugarBeet & Sugar (VNIISS), Ramon, Voronezh

region

Presently, haploidy is a perspective method to obtain new starting

material. One of the main tasks, when inducing hapioids from unfertilized

ovules of sugar beet, is to increase the rate o f regeneration.

To stimulate the regenerative ability of sugar beet unfertilized ovules,

stems o f donor material were exposed to high and low temperatures and x-

rays.

The results of the performed research showed, that x-rays gave the

best stimulating effect upon haploid induction. Quantity of the regenerants

was 7.37% with radiation dose being 3000 R. Doses o f 1000 and 2000 R

produced less effect, namely, 3% and 5%, respectively. In control variant

(without treatment), the regenerants were not formed. When temperature

treatments were used, the quantity of regenerants depended on the duration

of the treatment. Increase of haploid obtaining up to 3.4% and 4.5% was

observed when a low temperature (+4°C) treatment fo r 1-2 days was used.

Further increase o f the treatment duration (3days) led to decrease o f haploid

obtaining to 2.5%. Effect of a high temperature (+27.5°C) treatment fo r one

day stimulated development of 3% haploid regenerants. Stems being treated

for 2-3 days, therewas observed decrease of regenerative ability and increase

o f the quantity o f infected explants.

Thus, the most effective induction o f sugar beet haploid plants was

caused by treatment of generative organs with x-rays.

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