ЭлБиб

H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

MORPHOGENESIS FEATURES OF PLANTS FROM GENUS

CATTLEYA

LINDL.

CULTIVATED

IN VITRO

T.M .Cherevchenko, A.N.Lavrentyeva, Buyun, L.A.Kovalskaya

M.M. Grishko Central Botanical Garden, National Academy o fSciences o f Ukraine.

Timiryazevska St. 1, Kyiv - 252014 Ukraine. E -mail: cherevchenko@botanical

D ifferent method of clonal plant m icropropagation of 5 genotypes o f

Cattleya Lindl. in vitro

culture were studied. Young growing shoots 10-15 cm

in height were selected for propagation. As initial explants apical and lateral

shoot were used. The buds o f basal part of shoots have the highest

morphogenetic potential. After 6 months o f cultivation in nutrient medium 70

protocorms were formed, while o f the middle part o f shoot formed not more

than 10-15 protocorms. We determined that apical meristem of young shoots

o f all genotypes is inert under cultivation

in vitro.

It doesn’t develope. Explant

ability to regenerate depends on phase o f plant-donor development. Buds

found in may-june form protocorms more intensively and quickly, this is

provoked by increasing of phytohormonal complex activity of plants in this

period. We used different modifications o f nutrient media fo r cultivation o f

cattleya’s explants. Optimal medium fo r protocorm proliferation was M-S

w ith 5 mg/l BAP, 2 mg/l NAA, 100 mg/l peptone, 15% coconut milk, 1.5 g/

1 activated charcoal. More intensively protocorms were formed in darkness.

The most active zones of protocorm formation are bases of leaf primondiums

and bud squamules. As a rule 4-5 meristematic centres with lots o f protocorms

form simultaneously, they can be devided and cultivated. It must be marked

that the more complex hybrid is, the harder in introduction in culture and

protocorm formation.

Effective method of cattleya micropropagation is induction o f protocorm

formations on leaves of plant-regenerators and seedlings. Leaves are carefully

separated from stem and cultivated on M-S with 2 mg/1 BAP, 0.3 mg/1

NAA, 15% coconut milk. A fte r one month at the base of leaves, at the first

from epidermal tissues forms numerous of protocorms, later shoots.

Thus, combining some methods of micropropagation we can get planting

material of these beautiful ornamental plants.

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