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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A TIO N
RHIZOGENESISOF
SYRINGA VULGARIS L I N VITRO
E.A.Popowich,N.V.Getko
Central Botanical Gardens ofByelorussian NationalAcademy ofSciences, Minsk,
Byelorus
T h e re a re som e o p in io n s o f n e c e s s a ry o f ro o tin g s ta ge in
micropropagation of lilacs. However, in practice the period of growth inhibition
has increased in 2-3 time during adaptation ex
vitro
o f shoots w ithout roots
and it can led to production o f slowly growing plants. So we hold that the
rooting stage
in vitro
is expedient.
Micropropagated shoots o f lilac cultivars «Lunniy Svet» and «Nesterka»
1.5-2.0 sm long were used in the examine. They were placed on three variants
of nutrient media:
N1. Half N2. mineral salts concentration MS medium, hormone free;
N2. Full mineral salts concentration MS medium, enriched with 0.1
mg/l naphtylacetic acid (NAA);
N3 Half mineral salts concentration MS medium, enriched with 0.1 mgl-
1 NAA.
All the media containing thiamine, pyridoxine and nicotinic acid 1mg/
1,2% sucrose and 0.7% agar (Bacto Difco). Root formation had begun after
10-12 days. A fte r 4 weeks the amount o f rooted shoots and the number o f
roots per single shoot were evaluated.
The best results were received on the N3 medium for the both cultivars.
The amount of rooted shoots was 79.4±10.4% in «Lunniy Svet» culture and
96.0±2.0% in «Nesterka» culture on this medium. Analysis of the results
show that the both decrease in mineral salts consist and addition o f NAA
had influenced on the rhizogenesis o f lilacs.
Also a tendency to increase in the average number o f roots per shoot
was observed after cultivation from 1.5±0.2 on the N1 medium to 2.2±0.4 on
the N3 medium in «Lunniy Svet» culture and from 1.8±0.2 to 2.0±0.1 in
«Nesterka» culture, respectively, but differences between these data were
not significant. Perhaps, the increase in the number of roots resulted from
the better conditions o f taking root.
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