ЭлБиб

H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

EFFECTOFSUCROSEONFORMATIONCORMSOFCROCUSAND

BULBSOFHYACINTH

IN VITRO

E.A.Popowich,N.V.Getko

Central Botanical Gardens o fByelorussian National Academy o fSciences, Minsk,

Byelorus

It is often used cytokinins for multiplication in present micropropagation

technology of bulbous and corms plants.The increasing the number of shoots

and bulbs result in their diminution. Therefore the problem of additional growth

o f bulbs and corms to 4-5 mm and more so that may increase in their

viab ility during growing

ex vitro

is arise. The goal of the work was studying

influence o f different sucrose concentrations in nutrient medium on this

process.

Shoots of Hyacinthus orientalis cultivar «edisson» about 1.2-2.0 mm in

diameter were placed on the nutrient media MS, containing 2 ,4 ,6 ,8 or 10%

sucrose. The first 8 weeks the shoots were maintained under 21±1°C in

light, the following 20 weeks under 3±1 °C in dark and then 10 weeks under

25±1 °C in light. Shoots of Crocus ve rnus cultivar «Paulus Potter» were

cultivated on the nutrient media MS, containing 3 or 8% sucrose under

18±2°C in light during 12 weeks. The diameter o f receipt bulbs and corms

were evaluated at the end o f the experiments.

The results o f the both experiments have show a tendency to increasing

o f bulb and corm in diameters under raising sucrose concentration. Bulbs o f

hyacinth on the media enriched 2 or 4% sucrose had already significant

difference in diameter and were 3.9+0.1 mm and 4.4±0.2 mm, respectively.

Bulb expansion was observed to 8% sucrose in medium (4.9±0.2 mm) and

the diameters were significantly different at P<0.01. Increasing sucrose

content to 10% did not enlarged bulbs.

Expansion o f crocus corms have took place under increasing sucrose

content in nutrient media from 3 to 8% as well, and corms were 3.2±1.4 mm

and 4.7±0.7 mm in diameters respectively.

Perhaps, the mechanisms o f formation and growth o f bulbs and conns

in vitro

are the same in the different plant species.

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