ЭлБиб

H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

EXPERIENCE OF INTRODUCTION INTO

IN VITRO

CULTIVATION OF

REJUVENESCENT SELECTION MATERIALOF

QUERCUSROBUSL.

M.Y.Necliaeva, L.M.Burdaeva

Research Institute o fFores Genetics and Breeding, Lomonosov str., 105, Voronezh,

Russia, 394043

E-Mail:ilgis@lesgen.voronezh.su

Lately biotechnological methods fo r reproduction o f genetically valuable

plants (older than 15 years) a re applyed after the selection estimation have

been caried out. Rejuvenescent oak plants from clonal and family plantations

(15 and 20 years) have been used during experiments. Our goals were

determination o f oak ability fo r morphogenesis and regeneration

in vitro.

As the cultural explants there were used stem sections w ith the buds

obtained from root and stump sprouts. Into

in vitro

culture we introduced

buds o f sprouts w ith different lignification degree. Cultivation media were:

MS,WPM ,WTM ,E w ith BAP and GK3(0,1-1 mg/l) added.

It has been stated, that morphogenic activity o f axillary buds o f green

sprouts is higherthan o f renewal buds. Morphogenic cultures number in the

May-July experiments was 31,2-76,6%, multiplication coefficient was 1-8,

in some experiments this index reached 15. Renewal buds proved to be

unfit fo r

vitro cultivation and vitality coefficient made up 3-4% during these

experiments. Comparison o f morphogenic effectiveness in the explants of

different origin has shown that stump sprouts have the highest morphogenic

activity. The number of cultures which has begun their development reached

70%, multiplication coefficient was 5,3. The greatest morphogenic activity

has been registered on the media BTM and WPM with BAP ( 0,2-0,6mg/l)

and GK3 (0,1 mg/l). V itality o f induced sprouts kept during 6 months.

Thus, shoots o f sprouting adult oak trees have sufficient morphogenic

activity, realizing under certain cultivation

in vitro

conditions and isolation

tim e . They may be used as initial material fo r clonal improved forms o f oak

micropropagation.

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