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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

OBTAININGOF NEW PSTV-FREE LINES OF POTATO PLANTS OF

RUSSIAN CULTIVARS BASING ONMODIFIED TECHNOLOGY FOR

DIAGNOSTICS AND VIRUS ERADIATION

S.Musin, V .Bojko, A.Babosha

All-Russia Potato Research Institute,KorenevоMoscow Region

Eradiation o f viruses through tradional tip meristem method does not

garantie eradiation o f PSTV. S tock plants in gene banks obtained from tip

meristem are,as a rule,virus-free, but they are not obviously PSTV-free, as it

can survive after traditional chemical and thermotherapy, inactivating viruses.

To determ in wether it is possible to eradiate PSTV effect of low temperatures

was studied both with and w ithout nucleases. The effect was studied upon

tip meristem culture, tube plants and tubers. PSTV detection in regenerant

plants was perfermed by three methods:1 .indicative plants; 2. lectrophoresis;

3 .m o lecu la r hybridization. Our technology o f eradicaton included several

new stages: 1.Tuber seedlings selected fo r obtaining o f healthy clones were

tested fo r PSTV by available methods; 2.P!ants obtained from indexes

characterized as "healthy" by the first PSTV test were tested again after

PSTV provocation (illumination and high temperature(30-35 degrees C); 3.Tip

meristem , obtained from plants, characterized as PSTV-free after first two

tests, was cultivated

in vitro

w ith application of several therapy elements

(low 2°C) temperature, nuclease addition into the medium); 4.P lants being

grown

in vitro,

in glass house and in field were tested three times by three

independent methods. As a result of PSTV tests (including those performed

by Agd ia company, USA) it was shown that new lines o f cultivars Udacha,

Lukjanovsky, Newsky, Pushkinets, Lugowskoj and others are PSTV-free.

The development o f a new, PSTV-free collection o f genotypes has been

started fo r the cultivars, included into State Register of Russian Federation.

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