ЭлБиб

H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

CULTURES

IN VITRO

OF SOME CACTUSES

P.A.Karpov, V.M.Novikova

State Nikita Botanical Garden UAAS, Ukraine, Yalta

The representatives o f fam .Cacfaceae, the spesies

Ariocarpus,

Astrophytum, Espostoa, Hamatocactus

etc. are characterized by a long,

asynchronous period o f seed germination (about one year and more) and

slow growth o f seedlings -1 - 1 ,5sm fo r one year.

Are devising and improving the receptions of cultivation cactuses of

these spesies

in vitro

in connection with genofond protection, maintenance

o f genetic cleanliness o f species, opportunity o f induction callus and

regenerant formation, propagation of plants.

In culture

in vitro

from seeds o f different stages o f development isolated

from th e un ripe fru its

A strophytum capricorne, Espostoa lanata,

Hamatocactus setispinus on various nutritious mediums are brought up viable

seedlings. From mature

Ariocarpus retusuz

seeds on MS medium (1962)

within one year the plants o f height 0,6 - 0,7sm, and root 1 - 1 ,5sm in length

were developed. With cultivating seeds

Astrophytum capricorne

in conditions

of same medium, completed 6-BAP and 2,4-D simultaneously was observed

as development o f seedlings and proliferation. Received callus differed on

morphology and colouring: light with the transparent not painted cells, yellow

and pink cells. Is allocated morphogenous callus, with flat dense mat-white

surface, with differentiated морфогенными by structures.

In cu ltu re iso la ted , dense p a le -p in k buds 1 -1 ,5sm in leng th

Gymnocalicium m ihanowichii var. Rubra.

from sepals is received callus,

subm itted by transparent colourless and pink cells.

4 2 8

Научная электронная библиотека ЦНСХБ