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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

CLONALMICROPROPAGATIONOFJERUSALEMARTICHOKE

(HELIANTHUS TUBEROSUM)

ANDMICROTUBERFORMATION

K.Z.Gamburg, L.V.Gamanets,E.F.Vysotskaya

Siberian Institute o fPlant Physiology andBiochemistry,

Siberian Branch o ftheRussianAcademy ofSciences, 664033, Irkutsk, P.O.Box 1243,

RUSSIA

Shoot culture o f jerusalem artichoke was initiated from the lateral shoots

o f field-grown plants. The culture was maintained and propagated by

microcutting of the onestemmed plantlets. A single microcutting with 2 lateral

buds produced a p lan tle twith 3-4 pairs of opposite leaves after 1 month of

cultivation. Thus, the number of plantlets could increase 3-4 times each

month. Very seldom (5 cases for 3 years of cultivation) plantlets bearing 3

leaves in each node instead of normal 2 opposite leaves appeared. These

plants could be maintained fo r a long time by the monthly transfer of apical

segment to fresh medium. However, it was impossible to propagate them

because the ir lateral buds produced shoots with 2 opposite leaves. The

plantlets transferred to soil and grown in a greenhouse or at field continued

to produce 3 leaves in each node.

The microcuttings (except apical one) transferred into the medium with

8 per cent sucrose and 0.5 mg/I BAP formed microtubers (50-200 mg

a fte r 1.5-2 months of cultivation in darkness at 18-20°C. The microtubers

contained up to 30 per cent dry weight of which inuline amounted more than

90 per cent. The microtubers germinated after 3-4 months of cold storage

(+3-4°C).

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