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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

IN VITRO

PROPAGATIONOF

BRACHANTHEMUMBARANOVII

(KRASCH . E T POLJAK)

N.A.Vechernina, O.K.Tavartkiladze.

Botanical Garden ofAltay State University, Barnaul,

E-Mail

:biochem@biogeo.dcn-asu.

A problem o f genofond preservation may be decided by application of

biotechnology approaches. Elaboration o f micropropagation methods of rare

and disappear plants allow realizing their potential to propagation in short

time.

Brachanthemum baranovii

(

Krasch. e t Poljak.)

(

Asteraceae) -

endem ic and dangerous species o fAltay wild flora. Seeds o f brachantemum

was germinated in aceptic conditions. Some basal mediums were studied.

The best results were demonstrated on Gamborg Bs basal medium, added

with cytokinins. Numerous facts of our experiment prove that the induction

ofadventive shooting is achieved by 0,2 - 0,5 pM 6-benzylaminopurine or5 -

10 mM kinetin : approximally 30-40 shoots per one explant was obtained.

Cytokinins applied to basal medium in rather high concentrations had

vitrification effect. Rooting of shoots was induced by 3-5 pM indole-3-butyric

acid. A fter 4 weeks regenerated plantlets o fbrachanthemum had such sizes

: height o f the shoots - 3-4 cm and lenght of the roots - 4-5 cm.

4 0 4

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