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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N
MICROCLONAL PROPAGATION OF ONION (
ALLIUMСЕРА L .)
LINES
FORHETEROTHIS SELECTION
G.V.Belskaya
Belarus Research Institute of Vegetable Crops, 127aMayakovskySt. Minsk 220028
Belarus.
New
in vitro
methodes are widely used to accelerate the development
o f onion hybrids F1. The two-years regeneration time, the outbreeding and
therefore high heterozygous nature, inbreeding depression of lines make
useful the using o f tissue culture techniques. Cytoplasmic male-sterile lines
are used to breed onion hybryds.
In vitro
methodes have an importent part in
the development and maintance of male-sterile clones.
M icroclonal propogation methodes of onion male-sterile lines are
developed. The initial explants are the parts o f immature inflorescences.
Surface is sterilized in sodium hypochlorite for 10 min. At first explants are
placed on the bulb-induction mtdium M.-S. supplemented with BAP (4 mg/
+l) and NAA (4 mg/l) fo r 2 monthes, then explants replaced on the shoot-
induction medium with IBA (1 mg/l) fo r 3 monthes. As the result about 2-3
m icrobulbs (till 8) develop in each tube. A ll cultures are maintained under
conditions 16-h photoperiod, 12-15 th. lux intensivity light, at 20-22 C.
Adaptation o f m icrobulbs takes place on the peat "Dvina" or the ion
exchangeable nutrient substrate (Inst. Physics, Bel. Acad. Sci.). Annualy
we m icropropagate 8-10 onion male-sterile lines and obtain about 600
microbulbs. Plants are involved in heterothis breeding process.
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