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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

APPLICATION OF BIOLOGICALLY EFFECTIVE SUBSTANCES TO THE TIP

MERISTEM CULTUREWHILE OBTAININGVIRUS-FREEPOTATO

V.V.Bojko, S.M .Musin

All-Russian PotatoResearch Institute,Korenevo, MoscowRegion.

To increase effectivity of obtaining o f virus-free potato, the tip meristem

method was coupled with chemiotherapy. The following substances were

applied: 1.bacterial endonuclease (100000-200000 units per a liter); 2. bacterial

ribonuclease (100000-200000 units per a liter); 3. deoxyhexahydro-1,3,5-

tryazin (neo-DGT), (10 0 mg/l); 4. allylthioureidobenzoic acid (O-ATB), (1,0-

2,0 mg/l); 5. orthophenylureidobenzoicacid (O-PTB), (1,0-2,0mg/l); 6. ridostin

(dsRNA from S.cereviseae killer strain), (4-8 mg/l); 7.riphastin (dsRNA of

fage F6), (4-8 mg/l); 8. PE-26 (substance obtained from arachidonic acid),

(dissolved 1:1000000); 9.34-S (a modified form o f emystim with arachidonic

acid),(dissolved 1:100000); 10.emystim SHK (form34), ( dissolved 1:100000).

Investigations performed have shown that application o f biologically

effective virus-inhibiting substance resulted in lower concentration of viruses

in tube plants, but their effect was virus-specefic. For example, 34-S inhibited

X,S and M potato viruses, the effect was particularly noticeable when

concentration of the substance in the medium was 10-6. Whem emystim

was added into the mediumthe concentration was 27% less fo r PVX, 10%

less fo r PVS and 13% less fo r PVM in comparison to control plants. For

PE-26 decreasing of PVX, PVS and PVM concentration was 28%, 35% and

32% accordindly. When form 34 was present in the medium PVX concentration

was less by 45%, but PVS and PVM were equal to that of control plants.

An tivira l substance riphastin reduced PVX and PVS content in plants by

28% fo r the first passage and by 30% fo r the second passage. Ridostin

application resulted in less concentration of PVX, PVS, PVM and PVL. The

difference was 10-32% depending on the cultivar. Thus, the data obtained

show that application of biologically effective substances are very prospective

fo r obtaining of virus-free potato. The greatest effect was observed for bacterial

endonuclease and ribonuclease.

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