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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

THE USE OF

IN VITRO

CULTURE METHOD FOR PROPAGATION OF

MEDIGINALPLANTS

R.K.Baiburina, M.M.Ischmuratova, A.A.Zaripova.

Gontinuons destruction and slow restoration of populations of rare plant

species - sources o f valuable medicinal raw material show that measures of

protection and regeneration o f their gene pool are very actual. In addition to

the use of traditional methods such as, the establishment of preserves and

sanctuaries the cultivation o f these plants

in vitro

is necessary fo r solution

o f the problem.

Rhodiola iremelica Boriss., R. rosea L , Valeriana officinalis

L , Paeonia anomala L.

belong to rare species recordet in "Red book o f

Bashkortostan Republic" and "Red book o f USSR". Thickets of

Polemonium

coeruleum L .

are also not numerous because o f intensive store. Models of

micropropagation used in the investigations are proliferation of axillary buds,

producing o f callus tissue withe the following induction o f organogenesis.

The technology o f clonal micropropagation o f

R.rosea

and

R.iremelica

including explantation of initial tissue, properly micropropagation, rooting of

propagated shoots, propagation of regenerated plantlets for planting in opened

substiatum , introduction o f plantlets in nature, primary chem ical and

ensymatic estimation of their raw material is developed in laboratory. Selekted

optimal conditions fo r cultivation

in vitro

of both

Rhodiola

species are based

on study o f in trabud deve lopm en t, ana tom ica l and m o rphologica l

construention of buds, their hormonal status as well as on selectio of

com position and consistence o f nu trien t med ia and so il m ixtures.

Comparative estimation of raw material o f wild, untroduced in culture and

vitro

regenerated plants is conductifed. Primary pharmacological study of

tinctures from raw material of regenerated plantlets shows the possibility of

their use for preparation o f medecinal compounds of stimulating action. Mass

production o f

R.iremelica

piantlets proceeds with the aim o f reintroduction of

species in natural locations. Technology of micropropagation of

P.coeruleum

is being developed using the avobe scheme. High coefficient of multiplication

up to 160 shoot per one exsplant obtained. Experimentally-commercial plot

is beineg created nohich may be serve as a base fo rdevelopment of industrial

technology o f raising o f planting material. Sterile culture of

V.officinalis

and

P. anomala

is obtained. The conditions for different steps o f

in vitro

cultivation

o f these species are being optim ized now.

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