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C E L L C U L T U R E FO R P R O D U C IN G P LA N T S R E S IS T A N T T O B IO T IC A N D A B IO T IC
FACTORS
INFLUENCEOFBORONSURPLUSONGROWTHINGENDPHENOLIC
COMPOUNDSACCUMULATIONIN ALHAGIKIRGHISORUM CELL
SUSPENSIONCULTURE
A.Sh.Utarbaeva, O.A.Sapko, R .M.Kunaeva, M.A.Aytchozhin
Institut o fmolecular biology and biochemistry, Dosmukhamedov (Michurin) st., 86,
Almaty, Kazakstan, E-Mail
The action o f boron surplus on mass accumulation and content of
phenolic compounds was investigated. Na2B4O7x 10H2O was used as source
o f boron in concentration 2-100 times (0.15- 6g/l) more then normal
concentration.
Increasing of boron concentration in 2 times did not effect on the growthing
characteristics of suspension. During increasing of boron content in 5-10
times stimulanion effect on growth of cells was observed. The concentration
o f boron more then normal in 50-100 times lead to death of cells in very
begining of cultivation. High boron level in culture medium change content of
phenolic compounds. Increasing the boron content in 2 times decrease
phenolic compounds quantity on 14% and 5-10 times lead to insignificant
increasing (7%).
Changes o f quality composition o f phenolic compounds on different
levels of boron solanisation are studied.
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