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M O L E C U LA R B IO LO G Y O F PLA N T C E L L S IN V ITRO

USEOFMOLECULARGENETICMARKERSWHEN CULTIVATINGSUGAR

BEETPLANT CELLS

N VITRO

T.P.Fedulova, T.P.Zhuzhzhalova, O.A.Podvigina, I.N.Gorina

TheAll-Russian Research Institute o fSugarBeet & Sugar (VNIISS), Ramon, Voronezh

region

Successful application o f methods of tissue and organs culture in sugar

beet breeding makes the question o f revealing and using of molecular genetic

markers in biotechnological works actual.

Isozyme analysis o f donor genotypes and obtained regenerants was

carried out w ith the aim to determ ine the opportunities to use isozymes as

genetic criteria when culturing un-fertilized ovules and petioles o f sugar beet

in vitro.

It's found, that donor genotypes, heterozygous in isozyme markers,

formed the most haploid plants (64%) o f the total microclones obtained.

Isozyme control showed their 100% heterozygosity in loci Me-1, ldh-1, Gdh-

Initial plants and regenerants, obtained from them, when culturing

petioles on selec-tive media, modeling abiotic stresses, caused by salinization

and herbicide chlorsulphuron, were assessed in 6 isozyme loci (Me-1, Mdh-

1, Mdh-2, ldh-1, ldh-2, Pgd-1, Gdh-2).

Identity o f isozyme spectra of control and tested plants was shown.

There was revealed the modification in malic-enzyme of regenerants,

obtained from callus as additional isoforms with less electrophoretic mobility

o f the frequency o f 10%, that, probably, is a result o f mutations, affecting

genome.

Thus, isozymes can be widely used as molecular genetic markers in

different biotech-nological investigations.

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