340 / 592
M O L EC U LA R B IOLOGY O F PLANT C E L L S IN V ITRO
MITOCHONDRIALGENOMEOFCALLUSTISSUEOF
ACONITUM
LEUCOSTOMUMV.
A.M.Zagidullin,R.D.Valieva, A.G.Mardamshin
Department ofBiochemistry and Cytochemistry, Ufa Scientific Centre, RussianAcademy
o fSciences, 450054, Ufa, Prospekt o fOktyabrya, 69, Russia, E-
Callus tissue o f Aconitum leucostomum was initiated from the mature
embryos on the Murasige and Skoog nutrient medium . Callus was
subcultivated afte r every 20 days. Mitochondrial DNA was isolated from 3
years old ca llu s by using the method o f phenol dep ro te in iza tion .
Electrophoretic analysis of total and restricted mtDNA was carried out in 0,8
% agarose gel. Well known that the size of mitochondrial genome of higher
plants vary from 200 kb to approximately 2500 kb. In addition to high molecular
weight mtDNA, plasmid like molecules are present in mitochondria of many
species of plants. M itochondrial genome of callus tissue of aconitum
leucostanum consisted of only the geterogeneous population of high molecular
DNA, but plasmid-like DNA was not detected. The size of mitochondrial
genome of aconitum leucostanum was calculated by summing up the lengths
of restricted fragments of mtDNA digested by H indlll and BamHI enzymes.
Average size o f genome based on the dates obtained on the two enzymes
was equal to 410 kb. Restriction profiles of mtDNA of aconitum leucostanum
as well as of the most of mtDNA of higher plants, were characterized by
nonecvimolaryty o f separate bands Probably the more prolonged cultivation
o r alteration o f cultivation conditions may cause a change of number of low-
molecular DNAs, and this will tha tallow to detect them by the electrophoretic
analysis.
3 4 0
Научная электронная библиотека ЦНСХБ