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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
ACCUMULATION OFALKALOIDS IN TISSUE CULTURE R AUWOLFIA
SERPENTINA UNDERRADIOACTIVE IRRADIATION
N.V.Kirillova, M.G.Smirnova, L.A.Troitskaya, V .P.Komov
St.Petersburg State Chemical PharmaceuticalAcademy, 197376St.Petersbmg Prof.
Popov Str., 14, E-mail:
It is well known that alkaloid formation could change quickly under
influence o f stress factors. Cell culture Rauwolfia serpentina (strain K-27)
was exposed to ionizing radiation in doses 8 ,50 ,1 00 Gy. Irradiation in the
doses 50 ,100 Gy led to significant decrease of indoline alkaloid content in
the cells during all the period o f cultivation. For the dose 50 Gy intensive
biosynthesis o f alkaloids started at the 50th day o f cultivation and reached
maximum by the 70th day. However, this maximum was 47% comparing
with standard. The tissue irradiated in the dose 100 Gy had low rate of
biomass increase and perished quickly. Irradiation in the dose 8 Gy caused
intensive alkaloid accumulation at the earlier stage of tissue growth (the
25th day) with maximum at the 60th day. In particular, at the 50th day o f
culture growth indoline alkaloid content increased 2.2 fold compared with
standard. The second experiment series was conducted fo r a 70-day-old
culture in 4 h after irradiation. The radiation dose 0.5 Gy resulted in the
increase of alkaloid concentration (13%). The dose 8 Gy led to the increase
of total protein content (9%) and decrease of alkaloid accumulation (40.5%).
Irradiation in the dose 100 Gy was accompanied by the decline o f all the
parameters except AAE activity. In 24 h after 100 Gy irradiation increase o f
protein content (12%) was observed, and concentration of alkaloids was
only 8.5% lower standard value against 30.8% decrease in 4 h. These data
could point out the reversibility o f processes taking place in the cells under
irradiation.
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