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C E L L SE L E C T IO N
USING PHYTOPHTHORAINFESTANS PHYTOTOXIC METABOLITES FOR
POTATOCELLBREEDING
N.A.Zakharchuk, A.M.Zaychenko, T.N.Oleynik, A .A.Kuchko
Institute forPotato Research, Nemesahevo, Borodyanka district, Kiev region, 255740,
Ukraine, E-Mail:
ZabolotnyInstitute ofMicrobiology and Virusology, 154, Zabolotny StrKiev, 252143,
Ukraine.
Phytophthora infestans (Mont.) de Bary is a pathogen o f one o f the
most spreaded and harmful potato diseases.
A t present new methods side by side w ith traditional ones gain
importance in breeding process in Ukraine, one o f them is breeding at cell
level. We consider developing methods of Ph. infestans phytotoxic metabolite
(PTM) isolation, identification and using in breeding in vitro to be urgent.
In our research we used I-II xyz, 1234 races as well as local fungous
population, isolated into pure culture. Ph. infestans fungous culture was grown
on liquid nutrient Czapek's medium under stationary conditions on matrasses.
Follow ing fractions were obtained a fte r fermentation: protein fraction,
polysaccharide fraction, chloroform fraction from cultured liquor, chloroform
fraction from mycelium and lyophilized cultured liquor, which were tested for
metabolites activity on leaves of Lugovskoy resistant variety and Nezabudka
susceptible variety. Concentration o f substances made up 1%. Characteristic
reaction (brown spots with necrotic halos) was found on protein fraction
variant.
We perform our research with protein fraction of Ph. infestans fungus to
PTM obtaining w ith subsequent using them as selective agents.
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