ЭлБиб

C ELL SE L E C T IO N

CELLULARSELECTION OFWHEATRESISTANCETO GLUMEBLOTCH

DISEASE

Letliy Jose, E.A.Kalashnikova

Moscow agricultural Academy named afterK.A.Timiriazev Department ofBiotechnology,

Str. Timiriazevskerya-44, Block-16. E-Mail:ab5380@mai!.sitek.tiet

Initial material was mature and immature embryos o f 8 genotypes (

W W , 81S-10,91S-15, Enita, Moskovskaya-35, Saratovskaya-29, fortune,

Gaterin temps). To obtain the primary callus, isolated embryos were cultivated

in modified media MS, containing inosit 100 mg/l, asparagin 120 mg/l,

ascorbic acid 20 mg/l, sucrose 2%, agar 0,7% and also 2,4D 2mg/l. As a

selective factor utilized 14th day cultural filtrate (CF) of fungi

Septoria nodorum,

which was obtained by cultivating fungi in liquid nutrient media MS in conical

flask in shaker. Cellular selection were carried out in aggregated media,

containing CF in concentrations 5 ,10 ,1 5 , 20%. There was two scheme of

selection: 1 )4 passages in selective media, 1 passage without selective

factor, 4 passages in selective media. 2) cultivation o f callus in selective

media w ith increasing concentrations of CF from 5-20% w ith every

subcultivation (4 passages), 1 passage without selective factor and repeated

cultivation of callus in selective media with increasing concentration of CF

from 5-20%.

The obtained results allowed to exposure some phenomenon which

happened with callus independently from utilizing selection schemes. In the

first passage o f selection about 85% o f callus were formed with medium

density, bright yellow coloured, but in the further subcultivation 50% callus

were formed with brown coloure without any sign o f morphogenesis. Further

cultivation o f callus in a non selective media causes to rehabilitate the

proliferation activity. Repeated utilization o f selective factor helps to select

callus which are resistant to CF o f S. nodorum after 8 passages in selective

media. Regenerates were obtained from resistant callus o f mature embryos

genotype Enita, and callus o f immature embryos genotype-81 S-10.

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