ЭлБиб

SOM A CLONA L VARIATIONS O F PLAN T S AND CU LTURED C EL LS

MICROSATELLITEMARKERSAPPLIEDTOSTUDYTHEINSTABILITYIN

AWHEATSOMACLONALLINE

O.B .Dobrovolskaja, N.A.Omelianchuk, K.A.Salina, E.I.Gordeeva, E.B.Budashkina

Institute Cytology andGenetics, Novosibirsk, 630090, Russia, E-Mail:

One plant with speltoid spikes, 5A monosomic, occurred in pedigree of

regenerant from callus tissue of wheat near-isogenic line ANK12. This plant

(SC2- the second generation o f somaclones) was selfed and 5A monosomies

were chose fo r further selling.

There were not revealed significant morphological and chromosomal

abnormalities in SC3-SC4. In SC5-SC6 plants with variability in the following

traits appeared: speltoidness, the length o f vegetation period, the height.

Some of these plants have meiotic abnormalities, trivalents and tetravalents

with participation o f 5A chromosome.

For molecular-genetic analysis the plants with inactivated B1 gene, the

inhibitor of awns, was chose. This mutation was arose in SK7 and connected

with appearance o f awned spikes.

The several microsatellite markers located at different sites along the

long arm o f 5A chromosome were applied to analyse the chromosomal

rearrangement during regeneration and further reproduction o f a wheat

somaclonal monosomic line. The elimination of several PCR fragments

carrying by centromeric region of 5AL chromosome o f wheat near-isogenic

line ANK12 was revealed in SK8. Also, decrease in length o f 5AL telocentric

was observed by cytological analysis. The sim ilar deletion was present in

SK5-SK6 and absent in SK3-SK4. It correlated with data when instability of

several traits was appeared first. There were no changes of molecular and

genetic markers in interstitial part o f 5AL chromosome. Mutation or deletion

o f telomeric region bring about inactivation o f B1 gene in SK7.

Thus, increase o f 5AL chromosome instability during reproduction of a

wheat somaclonal monosomic line cause the deletion and mutation only of

telomeric and centromeric regions o f this chromosome.

The study was supported by grant from the Russian Fund of Basic

Research (RFBR 96-04-50101)

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