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SOM ACLONAL VARIATIONS O F PLAN T S AND CULTURED C E L L S
SOMACLONALVARIABILITYANDGENETICPOLYMORPHISM
OBSERVEDBYRAPD-TECHNIQUE
Z.G.Kokaeva;T.V.Petrova;V.K.Bobrova; A.M.Bagrova; A.V.Troitsky;
S.A.Gostimsky
Department o fBiologyand A.N.Belozersky Institute ofPhysico-chemicalBiology,
Moscow State University, Moscow, Russia, 119899
(tros@bioevol.genebee.msu.su)
Somaclonal variability is a strong increase of an inherited variability of
tissue culture cells in regenerated descendants. In a present study DNA
marker polymorphism was investigated by RAPD-method ( Random Amplified
Polymorphic DNA) among different pea callus cultures and regenerants. To
estimate the level of genetic divergency we used 10 pea cultivars and 10
mutants as a control group. Isolated DNA was analyzed by RAPD method
with eight 10-mer primers. Amplified fragments were scored from gel
photographs using the videodensitometer. Employing each of the primers,
about 25-29 informative amplified DNA bands were generated from all DNA
samples. The amplified fragments ranged in size from approximately 270 bp
to 2000 bp. RAPD patterns for each DNA sample differ both in the number of
bands and in their intensity, i.g., DNA samples were heterogeneous in
genome structure. We conclude that differences among somaclonal variants
are comparable to those observed among different pea cultivars. The level of
divergency identified by RAPD method among somaclonal variants is of one
order of magnitude with the one observed between pea cultivars.
This work was supported by Russian Foundation of Fundamental
Researches and Biodiversity program of GNTP, Russia
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