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S O M A C L O N A L V A R IA TIO N S OP P L A N T S AND C U LT U R E D C E L L S

USEOFGENETICMARKERS(RAPD,ISOENSYMES,STORAGE

PROTEINS) INANALYSISOFTHEBACKCROSSPROGENIESOF

BARLEY-WHEATHYBRIDSANDTHEIRSOMACLONES

L.L.Bildanava, E.A.Salina., L.A.Pershina,T.A.Pshemchnikova, N.P.Kalinina,

V.K.Shumny

Institute o fCytology andGenetics SiberianDepartment o ftheRussianAcademy of

Sciences, 630090 Novosibirsk, LavrentievAve, 10E-Mail

Self-fertile lines obtained by backcrossing (BC) barley-wheat hybrids

H.vulgare L .(H.v.) x T.aestivum L. (T.a.) and H.geniculatum All. (H.g.) x T.a.

with cultured wheat and somaclones induced from embryo callus cultures of

F6BC3 plants were studied using different genetic markers. These lines had

the morphological characters of wheat. Amplified DNA fragments specific to

cultured barley were detected in the genomes of 42-chromosome BC3F6

lines of H.v. x T.a. hybrids. Introgression of genetic material of barley into

these lines was confirmed by isozyme assays. Zymograms of esterase and

shikimate dehydrogenase in these hybrid lines were typical of barley. Analysis

of gliadin composition demonstrated redistribution of genetic material of

wheats involved in backcrossing for the production of the F6BC3 lines. These

signs remained in somaclones with changed morphological and physiological

characters. Some of the BC lines obtained from H.g. x T.a. were found to

contain storage proteins typical of barley and to lack gliadin, the marker of

chromosome 1A from wheat Pyrotrix 28 involved in the production of these

lines. The variability of isoenzymes were discovered among somaclones of

these hybrid combinations.

The present work was supported by the Russian Foundation for

Fundamental Research (96-04-49973).

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