ЭлБиб

197 / 592

PRO LO NG ED CULTIVATIO N OF SOM ATIC PLAN T C E LL
COMPARINGGROWTH PARAMETERS OF STEVIA CELL SUSPENSION
WHEN CULITVATING IN THE FLASKSANDIN THE FERMENTER
N.I.Bondarev, A.M.Nosov, *A.V.Kornienko
Timiryazev Institute o fPlant PhysiologyRAS, BotanicheskayaStr., 3 5 ,127276, Moscow,
Russia, E-Mail:
*Mazlumov SugarBeet andSugar Russian Research Institute, 396030, Ramone,
Voronege district, Russia
To reveal the possibility o f stevia cells cultivation in the fermenter we
investigated several growth characteristics of earlier obtained suspension
when cultivating in the air-lift fermenter and in the flasks on the shaker.
Stevia cells were grown under batch cultivation at 26°C. We used basic MS
medium supplemented with NAA (1 mg/l) and BAP (0.5 mg/l). Cell number,
fresh and dry biomass and cell viability were detected within the cultivation
cycle.
Growth curves were similar for both methods of cultivation. The highest
possible specific rate of the growth was 0.22 d a y1for flasks and it was 0.25
d a y 1fo r the fermenter. The minimum periods of biomass doubling were 3.2
day and 2.8 day accordingly. Dayly biomass yield in the fermenter was 0.51
g/l, bat this index in shaking flasks conditions was 0.49 g/l. Sucrose utilization
officiency o f the first method was 0.31 and that of the second one was 0.34.
Thus, we have demonstrated the possibility to cultivate stevia cell
suspension in fermenter. The main suspension growth characteristics when
growing in the air-lift fermenter demonstrated a slight increase in comparison
with the same ones in shaking flasks conditions.
1 97
Научная электронная библиотека ЦНСХБ