ЭлБиб

PROLONGED CULTIVATION O F SOMATIC PLANT CELL

MOLECULARMECHANISMSOF STRUCTUREORGANIZATION

ALTERATIONOFPLANTMI TOCHONDRIALGENOMEWHEN

CULTIVATED

VITROFORALONG TIME

A.G.Mardamshin

B iochem istry an d C ytochem istry Departm ent, Ufa Science Center, Russian

A cadem y o f Sciences, P ro sp ect O ctyabrya 69, Ufa, Russia, 450054, E-Mail:

Mitochondrial genome of higher plants is the most complicated among

eucaryotes. According to the hypothesis dom inating nowadays it is

represented by heterogenous population o f DNA circular molecules which

are formed as a result o f intra - and intermolecular recombination. In the

process of

in vitro

cultivation of cells of some plant species the difference of

population composition o f mt DNA molecules between

in vitro

and whole

plants was shown. The observed differences were explained by recombination

between mt DNA molecules and their copy number change. On the basis of

the data obtained when analysing the dynamics o f mt genome organization

variation o f pea cells cultivated

in vitro

for a long time and also mt genome of

whole pea plants which were grown under stress conditions it was concluded

that at least in pea the alterations took place mainly only due to the copy

number change of prior ring DNA molecules. Obviously recombinations took

place only at the definite stage of plant evolution which resulted in formation

o f a big set o f linking groups. In case of considerable alterations of external

and internal conditions of plant cell existence these or those classes of mt

DNA ring molecules will dominate.

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