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T O T IPO T EN C Y AND M O R PH O G E N E SIS O F PLANT C E L L S

IN V IT R O

STUDYOFMORPHOGENICACTIVITYOFISOLATEDLEAFEXPLANTS

OFWHEAT,

IN VITRO

CONDITIONS

ReghunathanPillai. Lethy Jose, E.A.Kalashnikova

MoscowagriculturalAcademy namedafterКA. TimiryazevDepartment

ofBiotechnology. Tmiiryazevskaya-44,Block-16. E-Mail:

Studies the capacity of the isolated wheat leaf explants to form

morphogenic callus depend up on the nutrient media. The work was carried

out with 5 genotypes (Moskovskaya-35, Fortuna, Enita, WW, Gaterin temps).

As the primary explant we used the basal part of the flag !eaf(in a size of 3-

5 mm) from sterilized 7th day germinates. Cultivation of leaf segments were

canted out in modified media MS, containing inositol 100 mg/l, asparagine

120 mg/l, ascorbic acid 20 mg/l, sucrose 2% and agar 0,7%. Studied the

influence of 2,4-D 2 and 3 mg/l for the callusogenesis and the combination

of 2,4-D 2 mg/l with zeatin 0,5 mg/l and 2 mg/l, and also NAA 0,5 mg/l and

BAP 0,5 mg/l for morphogenesis.

Established that the concentration of 2,4-D affected the capacity of leaf

explants to form callus. For the genotypes Moskovskaya-35 and Fortuna

optimum media was which contains 2,4-D 3 mg/l, for Enita, WW, and Gaterin

temps - 2 mg/l. In those conditions formed hard, green colored callus. By

transplanting the callus to the media fo r regeneration the best results were

obtained from the media which contains 2,4-D 2 mg/l and zeatin 0,5 mg/l. In

variants NAA with BAP more than 50% shows rhizogenesis. In this condition

regenerates were obtained only for Gaterin temps.

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