ЭлБиб

T O T IPO T EN C Y AND M O R PH O G E N E SIS OF PLANT C E L L S

IN V IT R O

MORPHOGENETICPOTENTIALOFMAIZEINBREDSWITHDIFFERENT

LENGTHOFVEGETATIVEPERIOD

G.R.Piralov

Institute o fGrain Farming UAAS 322027, Ukraine, Dniepropetrovsk,

Dzerzhinskyst. N. E-Mail:

anna@dma.dnepropetrovsk.ua

In the report the results of the study of morphogenetic potential of the

maize inbreds with different length of vegetative period are generalized. The

early-late inbreds ИК226, CM7, F2, ДК2/66, ИК178-6 (the length of vegetative

period - 90-105 days), middle-late inbreds ДК558, ДК165/403, ДК675, ДК415/

443 (115-130 days) and late inbreds M 017 and B73 (up 140 days) have

been studied. Calli were induced from immature embryos on the medium

with macro- and microelements of MS medium, with vitamins of D-medium,

silver nitrate (10 mg/l), L-proline (690 mg/l), casein hydrolysate (100 mg/l),

sucrose (20 g/l) and 2,4-D (1 mg/l). The reaction of each inbred in tissue

culture was specific. This was expressed in essential differences among

inbreds in accumulation of fresh and dry matter, in frequencies of morphogenic

callus establishment, quantitative and qualitative indexes of regeneration on

media with different concentrations of 2,4-D. If morphogenetic potential of an

inbred the evaluated with the combination of the properties listed herein, we

can draw conclusion, that the middle-late inbreds possess much higher

morphogenetic potential, than the early-late ones. In particular, if in the early-

late inbreds the regeneration process does not proceed further than the

formation primordia and coleoptiles 1 cm long, in the middle-late inbreds

ДК675, ДК415/443 and ДК165/403 most part of morphogenic calli formed

plantlets 5-10 cm high. A part of these plants of the first two inbreds grew

well on the nutritional medium, finished its vegetation and formed the

progenitors. The explanation of differences in morphogenetic potentials among

inbreds with different length of vegetative period should be searched for in

genetical and physiological peculiarities of inbreds.

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