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T O T IPO T EN CY AND M O R PH O G EN E SIS O F PLANT C E L L S IN V IT R O
INDUCTIONOFTH EMORPHOGENETICPROCESSESINANTHER
CULTUREOFINTERSPECIFICANDINTERVARIETALWHEATHYBRIDS
P.A.Orlov, L.V.Kholylyova, V.V.Ryzhkov, L.A.Dvlenok. A.Yatsevich. L.I.Kudelko
Institute ofGenetics and CytologyASB. 220072, Minsk, Belarus
E-Mail: igc%
Plant ability for induction of morphogenetic processes in anther culture
is widely used in fundamental and applied investigations. At the same time
the genetic control o f characters defining this processes is not studied
enough. The aim of this work was to study the peculiarities of the expression
of such characters as number of responding anthers, total number of
embryoids, number of albino and green regenerants in reciprocal hybrids of
first generation and their parental forms. Earlier obtained wide hybrid material
including more than 80 combinations from reciprocal crosses of soft and
hard wheat as well as T. aestivum with another Triticum species was used
as plant material fo r the investigation. Formation of embryoids and latter
regenerants has been induced in most studied forms. Comparative estimation
of the results showed that is some combinations interspecific and intervarietal
crosses remarkably change values of the investigated parameters in hybrids
of first generation as compared with parental forms. The effect could be both
positive and negative. Strong differences were observed also depending on
cross direction. For example no responding anthers were found in hybrids
between varieties Kitt and Ciano in direct combination (Kitt x Ciano). At the
same time 16,39% of responding antherswere found in reciprocal combination
that reliably exceeded both parents (Ciano -1,08%, Kitt -11,20%). In some
cases both hybrids and parental forms had similar trait values.
Thus, reliable reciprocal differences at interspecific and intervarietal levels
on characters defining wheat plant ability to morphogenetic processes
induction in anther culture have been revealed. This supports data about
possible involvement of the cytoplasmic genetic systems in determination
of these parameters.
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