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TO T IPO T EN CY AND M O R PH O G E N E SIS OF PLANT C E L L S IN V IT R O
THEEFFECTOFTHEGENESREGULATINGTHEIN VIVODEVELOPMENT
OFSHOOTS INARABlDOPSIS THALIANA (L.) ONTHEGROWTHAND
MORPHOGENESISOFTISSUECULTURES
I.P.Polyanskaja, T.A.Ezhova. V.V.Chub, E.M.Kof*
Biologicalfaculty, MoscowState University, 119599Moscow,Russia.
E-Mail:
*TimiryazevInstitute o fPlantPhysiology, 127276Moscow, Russia
The mutant Arabidopsis plants (lines K-122 and K-158) and the plants
o f ecotypes Blanes and Dijon from the collection of Department of Genetics
(Moscow State University) were used in experiments. The plants from the
line K-156 carried the mutation in the gene le-2 from the 1st Arabidopsis
chromosome and were characterized by a strong increase in the number of
stem nodes (from 30 in Dijon plants up to 71 in the le-2 mutants) and reduced
length of internodes. These characters were connected apparently with the
elevated sensitivity to auxin which was demonstrated for le-2 mutants. The
callus initiation from the leaf explants and the formation of the buds and
roots for the le-2 mutants were observed in the media with lower concentrations
of the NAA than fo r wild type plants. The fresh weight of 30-days callus
cultures was 1.5-2.5 times higher for the mutants than for wild type in all
tested concentration of NAA (0.01-2 mg/l) and BAP (0.05-1 mg/l).
The plants of the line K-122 have reduced apical dominance and
characterized by agraviotropic root growth. The reduced (2-3 times) content
of IAA was shown for the mutant plants by ELISA method. In all media
tested the mutant plants produced slow- growing callus cultures and failed
to develop shoots.
The effect of the genes regulating in vivo Arabidopsis plant development
on the in vitro growth and morphogenesis can be explained by their direct or
indirect influence upon the sensitivity and/or endogenic hormonal content.
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