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T O T IP O T E NCY AND M O R PH O G EN E SIS OF PLANT C E L L S IN V IT R O
PLANTREGENERATION FROMCOTTONAPICALMERISTEMS
G.B.Nasirova, E.G.Holmuratov, D.V.Krupene, M.N.Nasretdinova,
M.D.Yakubov, S.A.Djataev
Institute ofGenetics andExperimental Biology ofPlantsAS RUz, PO. 97, Tashkent 700
000, Uzbekistan, E-Mail
One of alternative ways of getting regenerants from isolated tissue,
which can be transformed by different ways is the regeneration of shoots
from plants meristem. Apical meristem were dissected isolated from 7 day
s te rile sh oo ts o f co tton v a rie ty C -6524 , re fe rrin g to spec ie s
Gossypium.hirsutum L. Fo rthe cultivation of isolated meristems mediums
were used, the basis of which was composed of the salts MS, vitamins B5
according to Gamborg, agar 0,7%, glucose 3%. Phytohormones: 2,4-D,
IAA, BAP, kinetin in concentration 0,05 and 0,1 mg/l and NAA -2,0 and 5,0
mg/l in different combinations were added to the medium fo r the growth
induction and development of meristems. On medium, containing IAA in
combination with kinetin or with BAP, cotton meristems at first started to
grow, then their growth gradually slowed down, forming shoots gradually
became black and perished. 2,4-D in combination with both cytokinins has
stimulated the formation of callus tissue. At planting of meristems on the
medium, containing NAA - 2 mg/l the main part of isolated meristems started
to grow and the formation of shoots has begun, inconsiderable part of
meristem has started to form callus tissue. The most optimal combination
o f hormones fo r cultivation of isolated cotton meristem were NAA - 5 mg/l
with kinetin and BAP, moreoverthe latter combination stipulated for maximal
shoot growth. A t more prolonged cultivation on this medium shoots has
started to form roots.
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