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TO T IPO T EN C Y AND M O R PH O G EN E SIS O F PLANT C E L L S

IN V IT R O

SROUTING

OF BARLEY IMMATURE EMBRYOS

IN VITRO

K.L itovkin,

S.Ignatova,

G.Bondar

Plant

Breedingand

Genetics Institute

o fUAAN,

Ukraine, Odessa, 270026

During the establishment of bailey tissue culture system we faced a

trouble connected with

in vitro

sprouting of immature embryos which

prevented these expiants from callus formation. The addition of 2,4-

dichlorphenoxyncetic acid in a great amount to nutritional medium has not

allowed to attain a desirable effect: a percentage of sprouting embryos in

some genotypes continued to be rather high. Thus an examination of

genotypic influence on immature embryos response to proposed culture

condition appeared to be important.

The spring barley cultivars Helios and Golden Promise were used in our

study along with isogenic lines of cultivar Odessky 100, which differed in set

of genes controlling morphological traits: hooded ( К ) and short-awned (ari-

a 12 ) lines. Immature embryos of parent genotypes and hybrid embryos

from the crosses "Helios x Golden Promise" and "K x ari-a 12", 1-2 mm in

length (10-11 days post anthesis, field-grown material o f 1997 year) were

placed upon MS medium supplemented with 24 mg/l 2,4-D, 0,1 mg/l ABA,

1 g/l casein hydrolisate, 200 mg/l inositol, and cultured in darkness at 25

°C. After 21 days in culture, each embryo with attached callus was harvested

and its shoot length recorded. Analysis of the data allowed us to suggest

that lower sensitivity to proposed 2,4-D concentration manifested in a strong

shoot growth (genotypes Helios, K, Odessky 100) is predominant over high

sensitivity (weak growth; Golden Promise, ari-a 12). The similar data were

previously published (Caligary et al., 1987) and showed that cultivar Golden

Promise among all examined genotypes contained the greatest number of

recessive alleles controlling mean shoot length

in vitro .

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