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TO T IPO T EN C Y AND M O R PH O G EN E SIS O F PLANT C E L L S IN V IT R O
DIAGNOSTICS OFEARLYMORPHOGENIC EVENTS IN UNIORGANIZED
GROWING WHEAT (TRITICUMAESTIVUM)TISSUE CULTURE
P.V.Lapshin, A.B.Burgutin, R.G.Butenko.
Timiriazev PlantPhysiology InstituteRSA, 35Botanicalstr.,
Moscow 127276, Russia. E-Mail:
It is well known that the most important condition for succesful cell
engineering is the high de-differentiated tissues capacity to produce shoot-
regenerants. Conventional methods o f Gram ineous cell-engineering
(especially for Triticum ssp.) are focuced on so called "embryogenic calli",
although their early indentification is rather contradictory. Therefore, while
working at three spring wheat genotypes of T riticum aestivum such as cv.
Tajognaya and Fotos and 592 NIISHCRNJ breeding lines, we payed special
attention to these genotypes capacity to form well indentificated morphogenic
structures. Further they produced shoot-regenetants as often as about 100%
frequency. So, we expect this phenomenon revealed by us to help us to
improve the efficiency of the manipulation with wheat callus tissues.
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