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TO T IPO T EN CY AND M O R PH O G EN E SIS OF PLANT C E L L S IN V IT R O
DIFFERENTLI GHT SPECTRUMINFLUENCEON CALLUSOGENESISAND
MORPHOGENESIS INWHEATTISSUECULTURE
1,2P.V.Lapshin. 1R.G.Butenko, :V .S.Shevclukha
1Timiriazev PlantPhysiology Institute, RSA, 35 Botanical str., Moscow,127276, Russia.
E-Mail:
2Moscow agriculturalAcademy namedafter K.A. TimiriazevDepartment o f
Biotechnology. Timiriazevskaya-44,Block-16.E-Mail t
In vitro cells and tissues cultivation is turned out to be the most
progressive method to improve the efficiency o f new plants form producing.
Wheat, as it is well known, is the main grain crop. So, because of rather low
shoot-regenerating percentage on the background of nearly 100% wheat
callusogenesis ,the efficient callusing and shoot-regenerating system is of
great interest. The subject of our investigations wasto reveal the influence of
different light spectrums, such as blue and red regions comparing to white
one and the control dark conditions, on mature and immature wheat ovules
capacities to produce calli and shoots. So, mature and immature spring
bread wheat (Triticum aestivum) ovules were cultivated in the callusogenic
medium (base mineral M-S medium supplemented by 2.5mg/l 2,4-D) under
16-hours illumination by the red, blue and white luminiscent lamp . The
results were estimated as the increase of callus biomass and the percentage
of calli producing apical structures. Tissue cultivation underthe white light
illumination resulted in the maximum rise of callus biomass. The second
place was taken by the control dark experiments, and the third - by the blue
one. The minimum callus biomass increase was noticed at the red-light
conditions. Therefore, different light spectrums had demonstrated various
effects on bread wheat morphogenesis: blue light induced the top percentage
of regenerating calli; the effect of white light was inferior. The worst
regeneration index was established under red light illumination. These results
were well reproduced in the four consequent experiments. So, it was proved
the ligh t spectrum to be o f great influence on callucogenesis and
morphogenesis in spring wheat (Triticum aestivum) genotypes. We indicated
the white light favored the dedifferent tissue growth, whereas the blue one
stimulated shoot regeneration. These data are well agreed with the analogous
findings in other high plants.
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