ЭлБиб

Аграрная наука Евро-Северо-Востока, №6(61), 2017 г.

Максимальная выработка противовоспа-

лительных цитокинов в крови крыс подопыт-

ной группы наблюдалась на пятые сутки α-Ф-

НО 870,8±0,24 нг/мл, 1 ИЛ β 29,78±0,21 нг/мл,

а затем организм приходил в физиологическую

норму с показателями α-ФНО 82,5±0,56 нг/мл и

1 ИЛ β 16,4±0,71 нг/мл.

Список литературы

1. Ежов М.Ю., Ежов И.Ю., Канко А.К. Нере-

шенные вопросы регенерации хрящевой и костной

ткани // Успехи естественного естествознания. 2015.

№5. С. 126-131.

2. Нагаев Б.С., НагаеваМ.Х., Камбачокова Э.А.

О роли цитокинов в регуляции иммунной системы

при инфекционных заболеваниях // Инфекционные

болезни: Материалы III Ежегодного Всероссийского

Конгресса по инфекционным болезням (Москва 28-

30 марта 2011 г.). 2011. Т. 9, Приложение №1. С. 260.

3. Осиков М.В., Симонян Е.В., Федосеев А.А.

Механизм влияния эритропоэтина на количествен-

ный состав лимфоцитов в крови при эксперимен-

тальной термической травме // Современные про-

блемы науки и образования. 2016. №2. С. 35-38.

4. Noh G., Lee J. Atopic dermatitis and cyto-

kines: the immunoregulatory and therapeutic implica-

tions of cytokines in atopic dermatitis Recent Pat In-

flamm Allergy Drug Discov 2012. №.6(3). P. 248-261.

5. Smith A.J. Cytokine and cytokine receptor

gene polymophisms and their functionality Cytokine

and growth factor reviews. 2009. Vol. 20. №1. Pp.43-59.

6. Terryberry J., Armstrong G., Sawyer J. SQI

diagnostic human cytokine 8-plex microarray // SQI Di-

agnostic. 2012. P. 1-4.

7. Tomoiu A., Larbi A., Fortin C. Do membrane

rafts contribute to human immunosensce? // Ann. N.Y.

Acad. Sci., 2013. Vol. 1100. P. 98-110.

8. Alvarez-Rueda N., Leprieur S., Clémenceau B.,

Supiot S., Sébille-Rivain V., Faivre-Chauvet A. Bind-

ing activities and antitumor properties of a new mouse/

human chimeric antibody specific for GD2 ganglioside

antigen. // Clin. Cancer Res. 2017. №13. P. 5613-5620.

9. Godwin J.W., Pinto A.R., Rosenthal N.A.

Macrophages are reguired for adult salamander limb

regeneration // PNAS. 2013. №110(23). Pp. 9415-9420.

10. Руководство по экспериментальному (докли-

ническому) изучению новых фармакологических ве-

ществ. / Под ред. Фисенко В.П. М., 2000. С. 228-231.

11. Raza K., Singh B., Singla S., Wadhwa S.,

Garg B., Chhibber S. Nanocolloidal carriers of isotreti-

noin: antimicrobial activity against Propionibacterium

acnesanddermatokineticmodeling.Mol.Pharm.2013.10.

P. 1958-1963.

12. Dinarello C. IL-1 in the pathogenesis and treat-

ment of inflammatory diseases blood. 2011. Vd. 117.

P. 3720-3732.

13. Реброва О.Ю. Статистический анализ ме-

дицинских данных. Применение пакета прикладных

программ STATISTICA. М.: МедиаСфера, 2002. 312 с.

Regenerating properties of new drug Dyalderon

Aziamov M.A.,

PhD in veterinary, head of laboratory,

Agalakova T.V.,

PhD in biology, senior researcher

North-East Agricultural Research Institute, Kirov, Russia

The article presents studies of the functional activity of different types of immunocompetent cells in the study of

regenerative properties of a new drug dyalderon. The study was performed in the laboratory, on the model of purulent

wounds in male rats of the Vistar line, in accordance with international requirements of humane treatment of laboratory

animals. The two groups of rats, 10 animals each, were used to accomplish this task. Animals under anesthesia were dis-

sected skin flap area of 20 mm

was applied to the surface of the wound. 1bn. microbial cells of Staphylococcus aureus.

Through the day the rats of the experimental group was administered with dyalderon at a dose of 30 mg intramuscularly,

and the animals of the control group Cefazolin topically at a dose of 1 g per wound, during for 8 days. The criterion

for the regeneration of the wounds were the time of appearance of granulation, epitelizaciei, the leukocyte count and

groups T and b-lymphocytes, the level of phagocytosis and of some cytokines and decrease microbial contamination by

Staphylococcus is less than 10

microbial cells per 1 cm

. Observation of the animals was carried out for 25 days. The

results obtained allowed to establish that dyalderon contributed to the rapid tissue regeneration and elimination of the

etiopathogenetic factors of the purulent inflammation of microbic cells, and necrotic detritus and accelerate epithelializa-

tion of wounds. This dyalderon

s action activated of ТН1 lymphocytes subpopulations, which stimulate the production of

inflammatory cytokines α-TNF and 1-ИЛβ. The maximum development of anti-inflammatory cytokines in the blood of

rats of the experimental group was observed on the fifth day of α-TNF 870,8±0,24 ng/ml, 1 IL β 29,78±0,21 ng/ml, and

organism

s physiological norm, was came α-TNF 82,5±0,56 ng/ml, and IL 1 β 16,4±0,71 ng/ml.

Key words:

granulation, dyalderon, immunity, rats, regeneration, cytokines

References

1. Ezhov

M.Yu

., Ezhov

I.Yu.

, Kanko A.K.

Nereshennye voprosy regeneratsii khryashchevoy i

kostnoy tkani

. [Unsolved problems of regeneration of

cartilage and bone tissues].

Uspekhi estestvennogo es-

testvoznaniya

. 2015. no. 5. pp. 126-131.

2. Nagaev B.S., Nagaeva

M.Kh

., Kambachoko-

va E.A.

O roli tsitokinov v regulyatsii immunnoy siste-

my pri infektsionnykh zabolevaniyakh

. [Тhe role of cy-

tokines in regulation of the immune system in infectious

diseases].

Materialy III Ezhegodnogo Vserossiyskogo

Kongressa po infektsionnym boleznyam (Moskva 28-

30 marta 2011 g.). Infektsionnye bolezni

. [Materials of

the III Annual Russian Congress on infectious diseas-

es (Moscow, 28-30 March 2011). Infectious diseases].

2011. Vol. 9,

Prilozhenie

no. 1. pp. 260.

Электронная Научная СельскоХозяйственная Библиотека