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TO T IPO T EN C Y AND M O R PH O G E N E SIS O F PLANT C E L L S

IN V IT R O

OVERCOMINGGENOTYPEINFLUENCEONCUCUMBERPROTOPLAST

CULTURE

V.P.Duplij, A.V.Lytovchenko, V.A.Sidorov

Institute o fC ellBiology andGenetic EngineeringNatl. Acad. Sci. o fthe Ukraine

Zabolotnogo 148,252143Kiev, the UkraineEmail:

Twenty years o f investigations carried out in the world have resulted

plant regeneration fo r a few cucumber (Cucumis sativus L ) genotypes only.

One can meet problems in the induction o f cell divisions and more often in

the stage o f obtaining whole plants.

In ou rwork mesophyll protoplast plating efficiency (PE) appeared to be

genotype dependent under conditions o f liquid culture medium and ranged

from 8 to 60%. PE was higher (8290%) fo r immobilized in Ca2+-alginate

protoplasts and the differences fo r various cucumber lines were statistically

indistinguishable. Somatic embryogenesis was induced fo r 5 gynoecious

lines only. Embryos o f 3 cucumber lines had a strong tendency to

dedifferentiation and secondary embryogenesis on the solid hormone free

MS medium but adding 0.1 mg/l ABA and 0.3-1 mg/l kinetin improved embryo

maturation and precipitated the whole plant obtaining. In the liquid MS medium

the phenomenon o f secondary embryogenesis was not observed but somatic

proembryos formed roots only, and it was necessary to stabilize culture

medium pH by 5 mM MES to obtain piantlels. A fte r 4-12 weeks from the

beginning of embryo maturation regenerants became morphologically sim ilar

to the plants grown

in vitro

from seeds.

This results allowed us to hope that protoplast culture conditions control

w ill make possible plant regeneration fo r more w ide range o f cucumber

genotypes.

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