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TO T IPO T EN C Y AND M O R PH O G EN E SIS O F PLANT C E L L S
IN V IT R O
ANTHERCULTUREMETHODINWHITEHEADCABBAGE
(BRASSICA
OLERAСЕA L. VAR.CAPITAТА)
HETEROSISBREEDING
G.V.Belskaya,T.V.Semashko
BelarusResearch Institute o f Vegetable Crops, 127a
Mayakovsky St. Minsk 220028 Belarus.
W hite head cabbage has a broad fie ld o f application as vegetable.
Homozygous lines are used to develop heterosis hybrids F1. The two-year
regeneration time and self-incompatibility make this process very long (8-12
years).
In vitro
technique such as anther culture is more effective to induce
haploid plant formation and follow ing doubled haploid plants.
We have used anther culture in production o f haploid regenerants from
cabbage. Local Belarus cultivars and hybrids F1 were used. The donor plants
were grown to flowering in a grown room under a 16-h photoperiod at 12°-
14°C. Anthers were surface-sterilized with a sodium hypochlorite fo r 10 min,
washed tw ice w ith distilled water. In anther culture anthers containing
m icrospores in the late uninucleate stages were placed on a solid (0,6%
agar) MS medium containing 6% sucrose, 2 mg/l BAP and 0,8 mg/l NAA.
The basic medium was supplemented w ith serine and meso-inositol. The
anthers were cultivated at 7 days at 35°C before being transferred to 25°C.
Haploid (about 10%) and diploid plants were obtained.
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