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TO T IPO T EN C Y AND M O R PH O G E N E SIS O F PLAN T C E L L S

IN V IT R O

THEELABORATIONOFSHOOTREGENERATIONMETHODSFOR

GENETICTRANSFORMATIONOFWILDSTRAWBERRY(FRAGARIA

VESCAL.)

N .V.Balokhina, *M.A.Kalyaeva,*Ya.I.Buryanov

Pushchino S ta te U n iversity *B ranch o f Shemya k in an d O vchinnikov

In stitu te o f B ioorgan ic C hem estry, Pushchino, M oscow region, 142292

E -M a il

ph el@ fib kh .serpu kh o v.su

The w ild strawberry (Fragaria vesca), that contains a diploid genome

(2n=14), represents an ideal model to study foreign gene expression in

Fragaria.

The method fo r shoot regeneration from leaf explants o fw ild strawberry

(Fr. vesca cv. Ruyana) has been developed.

The leaves o f plants obtained from seeds were used as the source of

explants. The lea f disks w ith excised proximal and distal parts o f the blade

and placed abaxial side up on the medium were the optimal explants for

shoot regeneration.

MS basal medium supplemented with 6-benzylam inopurin and indol-3-

butyric acid induced the maximum percentage o f shoot regeneration (70-80

%). The shoot regeneration process accompanied very low callus development

on the periphery o f the explants, so that organogenesis can be defined as

quasi-direct w ith a low risk o f somaclonal variation. 3 to 10 shoots were

regenerated on the leaf disks w ithin 6-8 weeks. Then shoots were isolated

and rooted in MS basal medium w ithin 2 weeks and the rooted shoots were

successfully acclimatized. The phenotypical variation was not observed.

We have a collection o f 6 varieties o f w ild strawberry

in vitro.

At this

tim e we are developing the methods to raise the percentage o f shoot

regeneration, and are carrying out the genetic transform ation o f various

species o f w ild strawberry.

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