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TO T IPO T EN C Y

a n d

M OR PHOG E N E S IS O F PLANT C E L L S

IN V IT R O

MASSOBTAININGOFSUGARBEETREGENERANTS

N.M.Bogomolova,E .E.Pauchenko, A.V.Kornienko

TheAll-RussianResearch Institute o fSugarBeet &Sugar (VNIISS), Ramon, Voronezh

region

Lack o f effective technologies o f mass regeneration in sugar beet is

one o f the main obstacles to introduce breeding materials into works on

genetic and cell engineering. As a rule, to make nutrient medium and regime

o f culture optimum does not enable to get desirable results. The performed

research allowed to suggest a principally new approach to solve the problem.

C ultivation o f a great number of plants

vitro leads to different kinds of

morphological alterations appearing in most genotypes. They are: alteration

o f lea f form , more th ick petiole, crimped edge o f lea f blade, alteration of

venation type, multiple shoot formation on adaxial surface o f petioles. Display

o f such a combination o f morphological alterations we marked as M-

phenotype. It was suggested, that these alterations are o f an epigenotypical

character, because regenerants o f M-phenotype retained the in itia l ploidy,

and, when transferred from sterile conditions to soil, they recovered the

morphological traits, that are characteristic to the in itia l forms.

A b ility o f petioles, obtained from plants o f M-phenotype, to adventitious

shoot formation was studied. On all the media tested (5 variants), regeneration

frequency was great - from 39.4 to 47.8%. Besides, most regenerants formed

not single soot, but several ones (from 2 to12). Regeneration o f adventitious

shoots was going according to the organogenesis pattern, mainly. However,

adding o f 2,4-D (0.4 mg/l) to the nutrient medium initiated direct somatic

embryoidogenesis. Explants formed em bryoidsw ith the frequency o f 70-

75%, and no inhibition o f organogenesis was observed.

Thus, petioles o f the leaves from plants o f M-phenotype are a very

perspective subject fo r biochem ical studies, because they have a great

morphogenic potential, that seems to be connected with no genetic changes.

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