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SECONDARY PRODUCTS OF CULTURED PLANT CELLS
SECONDARYMETABOLISM IN CULTURE
O F C .ROSEUS
Y.V.Yushkova, Y.V.Niconorova, S.M.Repyah, N.A.Velichko
Krasnoyarsk State technological academyMira st., 82, Krasnoyarsk, Russia 660049
One o f the most important questions o f plant biotechnology questions
about functions o f secondary metabolism in plant cell tissue culture.
The law o f secondary metabolism in the cells
in vitro
is different from
thus intact plants, what connected with specific cultured cells as artificial
biological (following to Nosov A.M.) as population of c e lls .
Determine o f space characteristics, in which exist the ceil population
have important population relationship in system "callus-substrate" and it
influence on secondary production.
In the laboratory o f biotechnology o f KSTA was studying influence the
method and conditions o f cultivation on production dimeric indole alkaloids
by the cell culture C. roseus.
The cultivation on agar, on polyurethane discs and in suspension culture
was investigated. The medium contained mineral basis by MS and NAA (10
mg/l) BAP (1 mg/l). Alkaloid yield was estimated by HPLC. Cultivation cell
culture on inert matrix (polyurethane disks) stimulated the formation somatic
embryoids and increased vinblastin and vincristin yield. It was 511,7 mg/g
d.w. and 33,5 mg/g d.w. respectively in compare with 330 mg/g d.w. vinblastin
and 11,5 mg/g d.w. vincristin on agar and 31,2 - vinblastin w ith absence
vincristin in suspension.
Increasing growth rate and production level connected probably with
several reasons.
Liquid medium provided more complete using substances to the cells.
R eticu lar structure o f polyurethane may consider as a model o f
secondary tissue plant organism with it mechanic functions.
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Научная электронная библиотека ЦНСХБ