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CYTOGENETICAL FEATURES OF REGENERATION PROGENY OF
EXPERIMENTAL PLANTS WITH DIFFERENT GENOME SETS
SINTHESIZED WITH USE OF
HORDEUMPROCERUMNevski
PendinenG.I.* , Chernov V.E. **, Hanina A.S.**
*
N.I.Vavilov Research Institute of Plant Industry, Bolshaya Morskaya, 44.
S.-Petersburg, 190000, Russia
** Biotechnological Centre, North-West Agricultural Institute"Belogorka".
Podbelskogo, 9. S.-Petersburg, Pushkin, 189620. Russia
Comparative study of chromosome numbers variability in regeneration progeni
obtained by means of micropropogation in vitro of experimental plants synthesized with
employment of
H.procerum
was carried out. Plants-donors for micropropogation was
cytogenetical stability forms (haploid
H.procerum(
n=3x=21) barley-wheat hybrids of
H.procerum
with
T.aestivum
(n=6x
=
42) and parent sample of
H.procerum
(2n =6x=42))
and
cytogenetical instability reciprocal euploid and aneuploid hybrids
H.vulgare
with
H.procerum(
n=4x=28, n=4x-l=27) with high degree of chromosome numbers variability
in sporogenic tissue ( under 80%of hypo-, hyper- and polyploid microsporocytes in single
plants) (Pendinen, Chernov, 1997). Immature inflorescens was cultivated in N6 medium
with 2 mg/12,4-D during 4 weeks for calluses growth. N6 medium without hormons was
used for regeneration. Plant with chromosome numbers wich was different than plants-
donors of explants (27%) andmixoploid regenerants (48%) was identificated in all familis
of regenerant of hybrids
H.vulgare
with
H.procerum
. Only 25% of regenerants of this
group had chromosome number of plants-donors of explants. All regenerants of
H.procerum,
haploid of
H.procerum
and
H.procerum x T.aestivum
hybrid had identical to
plants-donors of explants chromosome sets. It is possible that cytogenetical stability of
plants ih vitro culture plants-donors of cxplants
is
connecting with degree of cytogenetical
stability plants-donors of explants. Variability of chromosome sets in regeneration progeny
of
H.vullgare x H.procerum
hybrids is resultt of cytogenetical instability caused of
genomes intcractionn in hybrid genome. In vitro culture condition intensify of this
instability.
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