ЭлБиб

Effect of night break on tuber formation

in vitro

Solanum

tuberosum,

ssp.

andigena

T.N. Konstantinova, L .I. Sergeeva, S.А . Golyanovskaya, N.P.Aksenova

Institute o f Plant Physiology RAS, 127276, M oscow , Russia

In this w o rk a strictly photoperiodic line o f

Solanum tuberosum

, ssp.

andigena

forming

tubers only in short days w as studied. Recently it w as found that a night break (NB) fully

prevented tuber form ation o f this clone in

in vivo

experiments. T he aim o f the present study

w as to investigate w hether the N B reaction can be also reproduced in the course o f the

regulation o f tuber form ation in

in vitro

system w ith heterotrophic type o f nutrition.

Plants o f

Solanum tuberosum

, ssp.

andigena

w ere cultured

in vitro

for 1,5-2 months on

M S m edium supplem ented w ith 60 mg l-1 inositol , 0.5 m g l-1 thiamine, 0.5 m g l-1

pyridoxine, 6% sucrose and 0.8% agar. Three different photoperiodic treatm ents w ere

studied: a) short days (SD ) (14 h darkness, 10 h light); b) long days (LD ) (continuous light);

and c) night break (NB ) (as SD, but the dark period w as interrupted in the m iddle by 1 h

w hite light irradiation).

P hotoperiod influenced the m orphogenesis and the pattern o f grow th o f underground and

aboveground plant organs. T ubers w ere form ed only in SD variant and w ere absent in LD

and N B plants. Plants grow n in N B displayed many grow th characteristics intermediate

betw een SD and LD . Com parison o f the experim ents

in vivo

and

in vitro

show ed the

sim ilarity o f N B effect on tuber form ation and grow th pattern o f

Solanum tuberosum

, ssp.

andigena

plants.

T he presented resultes enable us to say that the developted

m odel for the

m orphogenesis o f potato plants

in vitro

may be used for the investigation o f regulatory

photoperiodic reaction in tuber formation.

- 5 3 8 ( 3 ) -

Научная электронная библиотека ЦНСХБ