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Chikrizova O.F., Poliakov A

All R ussian F lax R esearch Institute, T o rzh o k , T ver region

G en e-en g in eeric in v estig atio n s o f linseed a re w idly carried o u t in C a n ad a

(Jo rd o n M .C ., M c H ugh en A ., 1987; 1988; M cH u g h en A ., 1989), G re a t B ritain e

(B asiran N . e t a l., 1987) an d C zech R e p u b lic (M ly n aro v a L., P reto v a A .,1993).

H o w ev er sp ecific co n d itio n s a re reg u ared fo r flax tra n sfo rm a tio n becous o f low

m o rp h o g en ic cap ac ity in v itro .

H y p o co ty l segm ents o f flax cu ltiv ars D ash k o v sk y 2 an d B elinka w ere

co cu ltiv a ted w ith a d isarm ed stra in o f A . tum efaciens L BA 4404 (PB I 121) and A

281 (pT iB o 342) p G A 482. H ypocotyl segm ents o f 1-3 mm long ( B a siran N „ 1987;

D ra p p er J ., 1988), o r 8-10 mm in th e len g th an d w ith p a rtia lly rem oved (50

ep id erm is (M cH u g h en A ., 1989), an d also segm ents o f th e sam e length w hich w ere

p e rfo ra te d by a need le w ere used fo r th e tran sfo rm a tio n .

T h e n u m b e r o f m o rp h o g en ie ex p lan ts w ere v aried from 12 to 54 % in depend o n

siz e a n d ag e o f h y p o co ty ls. 7-8 days o ld flax explans h ad th e b est reg en eratio n

ab ility . M o rp h o g e n ic ex p lan ts in co n tro l m ad e up 71 % .

T h e co m p o sitio n o f n u trtio u s m edium also had a n influence o n reg en eratio n

p ro cess. In d u c tio n o f tran sfo rm ed bu d s an d sh o o ts o n M SB 5 2

m edium w as in 1,7

tim es h ig h er th an o n M S m edium .

I t w as d eterm in ed selectio n o f tran sfo rm ed flax ex p lan ts could be carried o u t

successefully o n m ed ia co n tain in g k a n a mycin (50-100 mg /l), c la p h o ra n (200 m g/l),

c o rb en icillin (200 m g/l).

V iable flax reg en eran ts w ere o b tain ed o n th e b aso s o f w orked o u t to ch n iq u e .

T ran sg en ic n a tu re o f th e reg en eran ts w as confirm ed by N P T -II ( neom ycin

p h o sp h o tra n sfe ra se II) activ ity .

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