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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

ENVIRONMENTALVARIATIONINVIRUS-FREEPOTATOPLANTS

I.M.Yashina, A.P.Glukhov

Russian Potato Research Institute, 140052, Korenevo, Luberetsty distr.M oscow region,

Russia Tel:557-50-01, ex.4-93

Virus-free plants of potato cultivars Belousovsky (B), Vestnick (V) and

Reserv (R) were produced by the conventional method o f

in vitro

apical

meristem excision. The environmental variation o f these plants m inituber

progeny was estimated during 4-5 vegetative generations. Virus-free lines of

cultivars В and R exceeded their initial forms by leafy tops growth, the stem

height, the size o f leaves and inflorescences, the number of tubers and late

maturation. It took them about 110-120 days to produce the potentional

tube r yield. They produced sm a lle rtubers after 95-days cultivating even in

the 4-th vegetative generation. Plants of cultivar В were characterized by the

capacity to set many small tubers w ithout stems height increasing. Studied

Jucovsky Runny virus-free lines demonstrated w ide mutation variation by

the tube r c o lo u r. We observed recessive white tubers in their vegetative

progenies. White tubers with coloured eyes were habitual as for line 1 as fo r

its precusor. Line 2 produced chimaeras white tubers. Line 3 demonstrated

poorer chimaeras phenomenon. Line 4 produced white tubers with vaster

eyes staining and coloured brow. Mutant line 5 varied by 7 traits, such as

white tuber skin, elongated tubershape, coloured brow, stainness, the number

o f tubers, thick pedicle with parthenocarpic berries and the later development.

The habit, leaves, flower and inflorescence features were not changed. We

consider such high mutation variability to have resulted from the application

of phytohormones for virus-free plants producing and the ir

in

vitro

preserving.

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