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H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

BIOREACTORASAGUARANTEEDWAYFORPOTATOMICROTUBER

MASSPROPAGATION

N.O.Yuorieva,A.N.Deryabin, A.V.Oreshnikov, R.G.Butenko.

Timiryazev PlantPhysiology Institute, Botanical sir. 35,127276,Moscow, Russia.

There is a "narrow" spot when virus free potato seed propagating. It is

the secondary v irus or viroid contamination. Therefore, to avoid this dangerous,

it is necessary to conducte the whole process o f potato microtuberization in

the strict sterile conditions. The bioreactor system is quite satisfied to these

requirements. We created the unique model of the bioreactorwith impulsive

nutrient medium spraying. Also, some suitable conditions fo r successful

potato m icrotuberization were found out. Stem explants were immobilized

on the specific net surface. Basing on the carried out experiments, we found

out the optimum way o f the cultural vessel loading, the necessary regimes

o f air, moisture and temperature conditions, and also, a succession o f nutient

medium changing. The analysis o f our experiments with

in vitro

potato stolon

cu ltu re resu lted in th e p o s s ib ility to regu la te the m ain stages o f

microtuberization such as mass stolonerifirization and tuber inducing. Using

this bioreactor model and the unique method of stolon cultivating, we obtained

a bulk o f m icrotubers (7-10 mm diameter) fo r two potato cultivars. These

tubers were characterized with nice size ranging and improved seed quality.

So, this experimental bioreactor and the method o f potato microtubers

producing are quite suitable fo r successful scaling up the process of virus

free potato microtubers propagation.

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