ЭлБиб

H E A L T H Y P L A N T S A N D M IC R O P R O P A G A T IO N

PHOTO SYNTHET IC APPARATUS FORMATION O F

GERBERA

H Y B R IDA

DURING M ICROCLONAL PROPAGATION AND UNDER

ADAPTATION

IN VIVO

Ju.Medvedeva. R.Karnachuk, A.Loginova

Department o fPlantPhysiology andBiotechnology, TomskState University, 36 Lenina

av., Tomsk634050Russia. E-Mail:

vaishlya@fbr.tsu.tomsk.su

Practical application of biotechnology methods fo rthe mass propagation

o f plants attaches great importans to the study o f lea f photosynthetic

characteristics

in vitro.

Culturing conditions are promote not only to the

effective micropropagation but also to the formation of normal photosynthetic

apparatus, so far as the survival o f regenerated plants depends on it.

Some structure - functional photosynthetic characteristics o f

Gerbera

hybrida

cv. Beauty during micropropogation stage and

in vitro

rooting stage

in d iffe ren t light intensity and also under plantlets adaptation after a

transference to soil were investigated. During

in vitro

culturing the influence

o f light intensity on leaf growth and chlorophyll content per unit o f area was

noted. Under adaptation

in vivo

chlorophyll content per unit of area in leaves

formed after a transference into the soil substrate was higher on 50%

compared to that , which was in leaves formed

in vitro.

Chlorophyll a/

chlorophyll b ratio in both cases was corresponded w ith the characteristics

o f lightdemanding plants. Leaf mesophyll differentiation in palisade and

spongy tissues occured at the age o f 9-10 days in leaves under micro-

propagation as well as in leaves

in vivo.

The cells o f palisade and spongy

mesophyll were differed by the chloroplasts number.

For

in vivo

formed leaves the more intensive increase of chloroplasts

and cells volume to be compared w ith

in vitro

formed leaves was shown.

However, the number of chloroplasts per cell and the number o f mesophyll

layers was the same. The obtained data are testified tha t a normal

photosynthetic apparatus is forming in leaves of

Gerbera

in process

in vitro

culturing.

4 4 0

Научная электронная библиотека ЦНСХБ