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CELL CULTURE FOR PRODUCING PLANTS RESISTANT TO BIOTIC AND ABIOTIC
FACTORS
INVESTIGATION OF ESTIMATION POSSIBILITY OF SPRUCEGAS
TOLERANTSINPLANT TISSUE CULTURE
VE.Chernov, N.E Fedorova
Forest Research Institute Institutskypr., 21.194021, S.-Petersburg, Russia, E-Mail:
Investigation o f possibility of estimation o f spruce to gas tolerance in
vitro culture was carried out for using Picea abies in town - planting o f North-
West populated localitis o f Russia and it's ear pollution. Spruce Picea abies
is cross polination species and it may gives genetical heterogeneous seeds
generation. This heterogenous (including gas tolerance index) may be observe
in early seedling growth. Growth chatacteristics of spruce callus tissue after
influence lethal and sublethal concentrations o f S 0 2 was observed fo r
investigation o f possibility estimation to gas tolerance in early stage o f
development. Spruce matture embryos was used to explantation on LP-
medium for obtaining calluses and seedlings. In first variant sulphurdioxide
fum igation in gas concentrations 0,35 and 0,75 g/m3 was used in 4 days
after embryos explantations, in second variant o f fumigation was used after
development intensive growth calluses (two month after explantation). Highest
tolerance in vitro to S 0 2 influense was oserved in calluses tissue. More
damage was observed in calluses wich cultivated in light condition then in
calluses wich cultivated in dark. Damage influence o f S 0 2was observed as
depress growth temps and development of intensive necrosis. Heterogenous
of growth indexis increase of intensive necrosis development with time later
after fumigation was obterned.
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