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PLANT GENETIC TRANSFORMATION. CELL SELECTION AND SOMATICHYBRIDIZATION
T-DNAINDUCEDALTERATIONSOFMORPHOLOGICALSIGNSAMONG
TRANSGENICTOBACCOPLANTS
A.A.Zagorskaya, E.V.Deineko,T.V.Novoselya, A.V.Romanova, A.YKochetov,
V.K.Shumnyi
The institute o fCytology and Genetics Siberian Department o fRussiaAcademy o f
Sciences Novosibirsk, 630090, e-mail:
In some times T-DNA insertions can change to development o f some
signs among transgenic plants. Such alterations are classified as T-DNA
induced mutations (Shell et al., 1992).
Transgenic tobacco plants (Nicotiana tabacum, SR1) with changed flower
morphology (longostily, mail sterility) were obtained.
The part of this plants in population has made 4,9 %. It is known that
signs connected with flower structures belong to conservative one from
evolutionary point o f view. Similar mutations are not revealed among 240 Ro
regenerated plants as well as 275 selfpollinated tobacco plants. It is possible,
that the morphological alterations of tobacco flower structure are connected
w ith T -DNA insertions. The close correlation between longostily and
kanamycin-resistance were demonstrated by genetic analysis.
Some others transgenic tobacco plants with different mutations (dwarf,
short period of development) were obtained.
The series o f genetic constructions permit to clone some genes
surrounding T-DNA insertions were created.
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