ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC

HYBRIDIZ ION

GENETIC TRANSFORMATION OF FLAX

(LINUM USITATISSIMUM L.)

M.A.Kalyaeva, *N.V.Balokhina, N.S.Zacharchenko, Ya.I.Buryanov

Branch o fShemyakin and Ovchinnikov Institute ofBioorganic Chemistry,

*Pushino Stale University, Pushchino, Moscow region, 142292, E-Mail:

The production of plants with new useful properties and resistance to

various pathogens is one of basic directions of modern plant biotechnology.

Now, flax is the object of genetical engineering, but each cultivar needs it,s

own individual conditions of transformation.

We transformed 4 domestic cultivarfiax: Belinka, Dashkovsky-2, Aleksin

and A-29. Segments of 7-10 day-old aseptic hypocotyls were used in capacity

of explants. The explants of flax were transformed with

Agrobacterium

tum efaciens

LBA4404 (pAL4404,pGA482) and

A.tumefaciens

LBA4404

(pAL4404, pGA482::M.EcoRII). The callus lines were grown on selective

medium MS, containing 1,0 mg/l 6-benzylaminopurine, 0,1 mg/l napthalene

a cetic acid 100 mg/l kanamycin fo r a long time. The e fficiency of

transformation depends on flax cultivars and

Agrobacterium

strain. The

e ffic ie n c y o f tra n s fo rm a tio n o f all c u ltiv a rs w ith

A .tum e fa c ie n s

LBA4404(pAL4404, pGA482::M.EcoRII) was less than in the case with an "

empty" vector.

The activity of neomycin phosphotransferase II was demonstrated for

kanamycin resistant callus lines (Km R). Molecular biological studies of the

obtained KmR callus lines are in progress.

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